EPDR1 levels and tumor budding predict and affect the prognosis of bladder carcinoma

Yue Yang; Hong Xu; Han Zhu; Dan Yuan; Hanchao Zhang; Zhengdao Liu; Faliang Zhao; Guobiao Liang

doi:10.3389/fonc.2022.986006

EPDR1 levels and tumor budding predict and affect the prognosis of bladder carcinoma

Front Oncol. 2022 Oct 7:12:986006. doi: 10.3389/fonc.2022.986006. eCollection 2022.

Authors

Yue Yang^{1

2

3}, Hong Xu¹, Han Zhu¹, Dan Yuan⁴, Hanchao Zhang^{1

2

3

4}, Zhengdao Liu^{1

3}, Faliang Zhao^{1

3}, Guobiao Liang^{1

3}

Affiliations

¹ Department of Urology, Affiliated Hospital of Zunyi Medical University, Zunyi, China.
² Department of Urology, Affiliated Hospital and Clinical Medical College of Chengdu University, Chengdu, China.
³ Medical College of Soochow University, Suzhou, China.
⁴ Department of Pathology, Affiliated Hospital of Zunyi Medical University, Zunyi, China.

Abstract

Background: Bladder carcinoma is a common malignancy of the urinary system. The previous study showed that EPDR1 expression was significantly related to the carcinogenesis and progression of bladder carcinoma.

Methods: We retrospectively reviewed the records of 621 patients who were newly diagnosed with bladder carcinoma between January 2018 and August 2020 at The Affiliated Hospital of Zunyi Medical University. We conducted immunohistochemistry of EPDR1 in tumor tissues. Meanwhile, tumor budding evaluation was also carried out by 2 independent experienced pathologists.

Results: 80 patients were included in this study with a median age of 66 years (range; 42-88 years). 45% of the patients (36/80) were non-muscle-invasive bladder carcinoma patients, while 55% of muscle-invasive bladder carcinoma(44/80). The follow-up time was from 6 months to 36 months. We found that there were significant differences in expression of EPDR1 in the tumor pT stages(p<0.05), pM stages(p<0.05), and pN stages(p<0.05). Meanwhile, a higher expression of EPDR1 indicated a worse outcome for the patient(p<0.05). A tendency toward a worse status of the patient was accompanied by a high positive rate (p<0.001). Moreover, the IOD of EPDR1 had a positive relationship with TB (p<0.05). Furthermore, we found that EPDR1 and tumor budding could be crucial factors for affecting the prognosis of bladder carcinoma, even better than pTMN(Riskscore=(0.724)* pT_stage +(4.960) *EPDR1+(4.312)*TB).

Conclusion: In conclusion, bladder cancer patients with higher expression levels of EPDR1 had worse survival outcomes. The combination of TB and EPDR1 levels could predict the prognosis for muscle-invasive bladder cancer patients.

Keywords: EPDR1; biomarker; bladder cancer; prognosis; tumor margin.