Fast calculation of spectral optical properties and pigment content detection in human normal and pathological kidney

Ana R Botelho; Hugo F Silva; Inês S Martins; Isa C Carneiro; Sónia D Carvalho; Rui M Henrique; Valery V Tuchin; Luís M Oliveira

doi:10.1016/j.saa.2022.122002

Fast calculation of spectral optical properties and pigment content detection in human normal and pathological kidney

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Feb 5:286:122002. doi: 10.1016/j.saa.2022.122002. Epub 2022 Oct 18.

Authors

Ana R Botelho¹, Hugo F Silva², Inês S Martins³, Isa C Carneiro⁴, Sónia D Carvalho⁵, Rui M Henrique⁶, Valery V Tuchin⁷, Luís M Oliveira⁸

Affiliations

¹ Physics Department, Polytechnic of Porto - School of Engineering (ISEP), Porto, Portugal.
² Porto University, School of Engineering, Porto, Portugal.
³ Center for Innovation in Engineering and Industrial Technology, ISEP, Porto, Portugal.
⁴ Department of Pathology and Cancer Biology and Epigenetics Group, Portuguese Oncology Institute of Porto, Porto, Portugal; Department of Pathological, Cytological and Thanatological Anatomy, Polytechnic of Porto - School of Health (ESS), Porto, Portugal.
⁵ Department of Pathology and Cancer Biology and Epigenetics Group, Portuguese Oncology Institute of Porto, Porto, Portugal; Department of Pathology, Santa Luzia Hospital (ULSAM), Viana do Castelo, Portugal.
⁶ Department of Pathology and Cancer Biology and Epigenetics Group, Portuguese Oncology Institute of Porto, Porto, Portugal; Department of Pathology and Molecular Immunology, Porto University - Institute of Biomedical Sciences Abel Salazar, Porto, Portugal.
⁷ Science Medical Center, Saratov State University, Saratov, Russian Federation; Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, Tomsk, Russian Federation; Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control, FRC "Saratov Research Centre of Russian Academy of Sciences," Saratov, Russian Federation.
⁸ Physics Department, Polytechnic of Porto - School of Engineering (ISEP), Porto, Portugal; Institute for Systems and Computer Engineering, Technology and Science (INESC TEC), Porto, Portugal. Electronic address: lmo@isep.ipp.pt.

PMID: 36274538
DOI: 10.1016/j.saa.2022.122002

Abstract

A fast calculation method was used to obtain the spectral optical properties of human normal and pathological (chromophobe renal cell carcinoma) kidney tissues. Using total transmittance, total reflectance and collimated transmittance spectra acquired from ex vivo kidney samples, the spectral optical properties of both tissues, namely the absorption, the scattering and the reduced scattering coefficients, as well as the scattering anisotropy, dispersion and light penetration depth, were calculated between 200 and 1000 nm. Analysis of the mean absorption coefficient spectra of the kidney tissues showed that both contain melanin and lipofuscin, and that 83 % of the melanin in the normal kidney converts into lipofuscin in the pathological kidney.

Keywords: Chromophobe renal cell carcinoma; Human kidney; Lipofuscin; Melanin; Renal cancer discrimination; Spectral absorption coefficient.

MeSH terms

Anisotropy
Humans
Kidney
Lipofuscin*
Melanins*
Scattering, Radiation

Substances

Melanins
Lipofuscin