Serial femtosecond crystallography approaches to understanding catalysis in iron enzymes

Curr Opin Struct Biol. 2022 Dec:77:102486. doi: 10.1016/j.sbi.2022.102486. Epub 2022 Oct 20.

Abstract

Enzymes with iron-containing active sites play crucial roles in catalysing a myriad of oxidative reactions essential to aerobic life. Defining the three-dimensional structures of iron enzymes in resting, oxy-bound intermediate and substrate-bound states is particularly challenging, not least because of the extreme susceptibility of the Fe(III) and Fe(IV) redox states to radiation-induced chemistry caused by intense X-ray or electron beams. The availability of novel sources such as X-ray free electron lasers has enabled structures that are effectively free of the effects of radiation-induced chemistry and allows time-resolved structures to be determined. Important to both applications is the ability to obtain in crystallo spectroscopic data to identify the redox state of the iron in any particular structure or timepoint.

Publication types

  • Review

MeSH terms

  • Catalysis
  • Crystallography, X-Ray
  • Iron* / chemistry
  • Lasers*
  • Spectrum Analysis

Substances

  • Iron