CRISPR-based point-of-care plant disease diagnostics

Tofazzal Islam; Shamfin Hossain Kasfy

doi:10.1016/j.tibtech.2022.10.002

CRISPR-based point-of-care plant disease diagnostics

Trends Biotechnol. 2023 Feb;41(2):144-146. doi: 10.1016/j.tibtech.2022.10.002. Epub 2022 Oct 19.

Authors

Tofazzal Islam¹, Shamfin Hossain Kasfy²

Affiliations

¹ Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh. Electronic address: tofazzalislam@bsmrau.edu.bd.
² Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh.

PMID: 36272820
DOI: 10.1016/j.tibtech.2022.10.002

Abstract

The coupling of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas RNA-programmable nucleases with nucleic acid detection platforms has brought radical changes to the field of disease diagnosis. Recently, Sánchez et al. developed a simple, rapid, highly sensitive, precise, and in-field deployable point-of-care (POC) and point-of-need (PON) molecular disease detection tool that can be used in diverse agricultural applications.

Keywords: CRISPR/bio-dCas9; lateral flow assay (LFA); nucleic acid detection; point-of-care testing (POCT); point-of-need testing (PONT); recombinase polymerase amplification (RPA).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agriculture
CRISPR-Cas Systems
Plant Diseases* / genetics