COMET Assay for Detection of DNA Damage During Axolotl Tail Regeneration

Belfran Carbonell; Jennifer Álvarez; Gloria A Santa-González; Jean Paul Delgado

doi:10.1007/978-1-0716-2659-7_12

COMET Assay for Detection of DNA Damage During Axolotl Tail Regeneration

Methods Mol Biol. 2023:2562:183-194. doi: 10.1007/978-1-0716-2659-7_12.

Authors

Belfran Carbonell^{1

2}, Jennifer Álvarez¹, Gloria A Santa-González^{1

3}, Jean Paul Delgado⁴

Affiliations

¹ Grupo Genética Regeneración y Cáncer, Institute of Biology, University of Antioquia, Medellin, Colombia.
² Department of Integrated Basic Studies, Faculty of Dentistry, University of Antioquia, Medellin, Colombia.
³ Biomedical Innovation and Research Group, Faculty of Applied and Exact Sciences, Instituto Tecnologico Metropolitano, Medellin, Colombia.
⁴ Grupo Genética Regeneración y Cáncer, Institute of Biology, University of Antioquia, Medellin, Colombia. jean.delgado@udea.edu.co.

PMID: 36272076
DOI: 10.1007/978-1-0716-2659-7_12

Abstract

The purpose of this chapter is to evaluate DNA damage during axolotl tail regeneration using an alkaline comet assay. Our method details the isolation of cells from regenerating and non-regenerating tissues and the isolation of peripheral blood for single-cell gel electrophoresis. Also, we detail each of the steps for the development of the comet assay technique which includes mounting the isolated cells on an agarose matrix, alkaline electrophoresis, and DNA damage detection.

Keywords: Alkaline comet assay; DNA damage; Tail regeneration; single-cell gel electrophoresis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ambystoma mexicanum* / genetics
Animals
Comet Assay / methods
DNA Damage*
Electrophoresis
Sepharose

Substances

Sepharose