A simulated toxic assessment of cesium on the blue mussel Mytilus edulis provides evidence for the potential impacts of nuclear wastewater discharge on marine ecosystems

Abstract

The toxic effects of cesium (Cs) on the blue mussel Mytilus edulis were experimentally investigated to assess the potential environmental consequences of the discharge of nuclear wastewater containing radionuclides. A simulated experimental system of stable cesium (¹³³Cs) was set up to mimic the impacts of radiocesium, and its heavy metal property was emphasized. The mussels were exposed to a concentration gradient of ¹³³Cs for 21 days, followed by another 21-day elimination period. ¹³³Cs exposure resulted in effective bioaccumulation with distinct features of concentration dependence and tissue specificity, and hemolymph, gills and digestive glands were recognized as the most target tissues for accumulation. Although the elimination period was helpful in reducing the accumulated ¹³³Cs, the remaining concentrations of tissues were still significant. ¹³³Cs exposure presented little effect on growth status at the individual level but had distinct interference on feeding and metabolism indicated by the oxygen consumption rate, ammonia-N excretion rate and O:N ratio, simultaneously with the impairment of digestive glands. Regarding hemocytes in the hemolymph, the cell mortality increment, micronucleus promotion, lysosomal membrane stability disruption and phagocytic ability inhibition suggested that the immune function was injured. The cooccurrence of reactive oxygen species overproduction had a close relationship with the observed damages and was thought to be the possible explanation for the immune toxicity. The assay based integrated biomarker response (IBR) presented a good linear relation with the exposure concentrations, suggesting that it was a promising method for assessing the risk of ¹³³Cs. The results indicated that ¹³³Cs exposure damaged M. edulis at the tissue and cell before at the macroscopic individual, evidencing the potentially detrimental impacts of nuclear wastewater discharge on marine ecosystems.

Keywords: Marine ecosystems; Mytilus edulis; Nuclear wastewater; Radiocesium; Stable cesium ((133)Cs).