Delivery of Mir-196c-3p with NIR-II light-triggered gel attenuates cardiomyocyte ferroptosis in cardiac ischemia-reperfusion injury

Jing-Jing Ji; Shang-Yu Chen; Zi-Wei Yang; Rui Zhang; Ling-Lin Qian; Yu Jiang; Jia-Qi Guo; Ya Wu; Qu-Li Fan; Yu-Yu Yao; Peng-Fei Sun

doi:10.1016/j.nano.2022.102618

Delivery of Mir-196c-3p with NIR-II light-triggered gel attenuates cardiomyocyte ferroptosis in cardiac ischemia-reperfusion injury

Nanomedicine. 2023 Jan:47:102618. doi: 10.1016/j.nano.2022.102618. Epub 2022 Oct 18.

Authors

Jing-Jing Ji¹, Shang-Yu Chen², Zi-Wei Yang³, Rui Zhang³, Ling-Lin Qian³, Yu Jiang³, Jia-Qi Guo³, Ya Wu³, Qu-Li Fan², Yu-Yu Yao⁴, Peng-Fei Sun⁵

Affiliations

¹ Department of Cardiology, Zhongda Hospital, Medical School of Southeast University, 87 Dingjiaqiao, Nanjing, Jiangsu 210009, China; Department of Cardiology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province 310009, China.
² State Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, Nanjing 210023, China.
³ Department of Cardiology, Zhongda Hospital, Medical School of Southeast University, 87 Dingjiaqiao, Nanjing, Jiangsu 210009, China.
⁴ Department of Cardiology, Zhongda Hospital, Medical School of Southeast University, 87 Dingjiaqiao, Nanjing, Jiangsu 210009, China. Electronic address: yaoyuyunj@hotmail.com.
⁵ State Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, Nanjing 210023, China. Electronic address: iampfsun@njupt.edu.cn.

PMID: 36270453
DOI: 10.1016/j.nano.2022.102618

Abstract

Ferroptosis plays an important role in ischemia-reperfusion (I/R)-induced cardiac injury and there are many defects in current targeted delivery of miRNAs for the treatment of ferroptosis. We herein report a unique hydrogel (Gel) that can be triggered by a near-infrared-II (NIR-II) light with deep tissue penetration and biocompatible maximum permissible exposure (MPE) value for in situ treatment after I/R. The mir-196c-3p mimic (mimics) and photothermal nanoparticles (BTN) were co-encapsulated in an injectable Gel (mimics + Gel/BTN) with NIR-II light-triggered release. Using 1064 nm light irradiation, local microenvironment photothermal-triggered on-demand noninvasive controllable delivery of miRNA was achieved, aiming to inhibit I/R-induced ferroptosis. Consequently, declined ferroptosis in cardiomyocytes and improved cardiac function, survival rate in rats was achieved through the controlled release of Gel/BTN mimics in I/R model to simultaneously inhibit ferroptosis hub genes NOX4, P53, and LOX expression.

Keywords: Ferroptosis; Gel/BTN; I/R; Mir-196c-3p; NIR-II.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Rats
Reperfusion Injury*