Highly sensitive genotyping of MTHFR C677T polymorphisms using a novel RPA-LDR-qPCR assay

Xinxin Si; Qinghua Gu; Chenjie Zhao; Xiao Zhang; Tingting Xiao; Yu Li; Wei Ying; Song Gao

doi:10.3724/abbs.2022151

Highly sensitive genotyping of MTHFR C677T polymorphisms using a novel RPA-LDR-qPCR assay

Acta Biochim Biophys Sin (Shanghai). 2022 Oct 25;54(11):1753-1756. doi: 10.3724/abbs.2022151.

Authors

Xinxin Si¹, Qinghua Gu¹, Chenjie Zhao¹, Xiao Zhang¹, Tingting Xiao¹, Yu Li¹, Wei Ying², Song Gao¹

Affiliations

¹ Jiangsu Key Laboratory of Marine Biological Resources and Environment, Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Co-Innovation Center of Jiangsu Marine Bio-industry Technology, School of Pharmacy, Jiangsu Ocean University, Lianyungang 222005, China.
² Department of Pathology, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China.

No abstract available

MeSH terms

Case-Control Studies
Genetic Predisposition to Disease
Genotype
Humans
Methylenetetrahydrofolate Reductase (NADPH2) / genetics
Polymerase Chain Reaction* / methods
Polymorphism, Single Nucleotide*

Substances

Methylenetetrahydrofolate Reductase (NADPH2)
MTHFR protein, human

Grants and funding

This work was supported by the grants from the Key Natural Science Research Project of the Jiangsu Higher Education Institutions of China (No. 20KJA416002), the Research Program of “521 Project” of Lianyungang City of China (No. LYG06521202133), the Open-end Funds of Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening (Nos. HY202004 and HY202105), the Postgraduate Research and Practice Innovation Program of Jiangsu Province (No. SJCX20_1240), the “Blue Project” of Jiangsu Higher Education Institutions of China, and the Priority Academic Program Development of Jiangsu Higher Education Institutions of China.