Activation of PTEN/P13K/AKT Signaling Pathway by miRNA-124-3p-Loaded Nanoparticles to Regulate Oxidative Stress Attenuates Cardiomyocyte Regulation and Myocardial Injury

Oxid Med Cell Longev. 2022 Oct 11:2022:8428596. doi: 10.1155/2022/8428596. eCollection 2022.

Abstract

As a common cardiovascular disease, acute myocardial infarction seriously affects the health and life of patients. miRNAs play an important role in acute myocardial infarction. Based on miRNA obtained from the previous sequencing, this study investigated whether miRNA (miR)-124-3p-loaded nanoparticles (NPs) affect the phenotype of the acute myocardial infarction (AMI) rat. Nano-miR-124-3p decreased the myocardial infarction area, improved the myocardial tissue structure, and increased the degree of fibrosis. Nano-miR-124-3p decreased apoptosis and the expression of cleaved caspase 3, indicating its role in protecting and repairing the myocardium. To further verify the action mechanism of miRNA, a potential target gene of miR-124-3p, PTEN was identified by STARBASE and further confirmed using double luciferase assays. Following cotransfection of nano-miR-124-3p and PTEN, the areas of tissue structure damage, myocardial infarction, and fibrosis were substantially elevated. The expression of cleaved caspase 3 and the apoptosis rate in the nano-miR-124-3p and PTEN cotransfection group was also significantly increased. Bioinformatics analysis revealed that miRNA-124-3 may regulate oxidative stress injury by targeting PTEN. Taken together, miR-124-3p could protect and repair myocardial tissues through targeting PTEN.

Publication types

  • Retracted Publication

MeSH terms

  • Animals
  • Caspase 3 / metabolism
  • Fibrosis
  • Luciferases / metabolism
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • Myocardial Infarction* / pathology
  • Myocytes, Cardiac / metabolism
  • Nanoparticles*
  • Oxidative Stress
  • PTEN Phosphohydrolase / genetics
  • PTEN Phosphohydrolase / metabolism
  • Proto-Oncogene Proteins c-akt / metabolism
  • Rats
  • Signal Transduction

Substances

  • MicroRNAs
  • Proto-Oncogene Proteins c-akt
  • Caspase 3
  • Luciferases
  • Pten protein, rat
  • PTEN Phosphohydrolase
  • MIRN124 microRNA, rat