Evaluation of cadmium tolerance and remediated efficacy of wild and mutated Enterobacter species isolated from potassium nitrate (KNO₃) processing unit contaminated soil

Haiying Lu; Changlei Xia; Arunachalam Chinnathambi; Omaima Nasif; Mathiyazhagan Narayanan; Sabarathinam Shanmugam; Nguyen Thuy Lan Chi; Arivalagan Pugazhendhi; Ruangwong On-Uma; Kumchai Jutamas; Wongchai Anupong

doi:10.1016/j.chemosphere.2022.136899

Evaluation of cadmium tolerance and remediated efficacy of wild and mutated Enterobacter species isolated from potassium nitrate (KNO₃) processing unit contaminated soil

Chemosphere. 2023 Jan;311(Pt 1):136899. doi: 10.1016/j.chemosphere.2022.136899. Epub 2022 Oct 17.

Affiliations

¹ College of Biology and the Environment, College of Materials Science and Engineering, Nanjing Forestry University, Nanjing, Jiangsu, 210037, China.
² Department of Botany and Microbiology, College of Science, King Saud University, PO Box -2455, Riyadh, 11451, Saudi Arabia.
³ Department of Physiology, College of Medicine and King Khalid University Hospital, King Saud University, Medical City, PO Box-2925, Riyadh, 11461, Saudi Arabia.
⁴ Division of Research and Innovation, Department of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Science, Chennai, Tamil Nadu, India.
⁵ Biosystems Engineering, Institute of Forestry and Engineering, Estonian University of Lifescience, Kreutzwaldi 56, 51014, Tartu, Estonia.
⁶ School of Engineering and Technology, Van Lang University, Ho Chi Minh City, Vietnam.
⁷ Emerging Materials for Energy and Environmental Applications Research Group, School of Engineering and Technology, Van Lang University, Ho Chi Minh City, Vietnam. Electronic address: pugal.smile@gmail.com.
⁸ Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, 50200, Thailand; Innovative Agriculture Research Center, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand.
⁹ Department of Plant Science and Soil Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand; Innovative Agriculture Research Center, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand.
¹⁰ Department of Agricultural Economy and Development, Faculty of Agriculture, Chiang Mai University, 50200, Thailand; Innovative Agriculture Research Center, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand. Electronic address: anupong.w@cmu.ac.th.

PMID: 36265702
DOI: 10.1016/j.chemosphere.2022.136899

Abstract

The purpose of this study was to find the most cadmium (Cd²⁺) tolerant and remediated bacteria isolate from KNO₃ processing unit contaminated soil. One isolate out of 19 isolates possessed excellent Cd²⁺ tolerance than others, which was recognized as Enterobacter hormaechei SFC3 through molecular characterization (16S rRNA sequencing). The identified E. hormaechei SFC3 contained 55% and 45% of GC and AT content, respectively. The wild and acridine orange mutated E. hormaechei SFC3 exhibited excellent resistance to Cd²⁺ up to the concentration of 1500 μg mL^-1. Furthermore, the wild E. hormaechei SFC3 and mutated E. hormaechei SFC3 showed 82.47% and 90.21% of Cd²⁺ remediation on 6th days of treatment respectively. Similarly, the Cd²⁺ tolerant wild and mutated E. hormaechei SFC3 showed considerable resistance to all the tested antibiotics. The findings indicate that E. hormaechei SFC3 isolated from KNO₃ processing unit contaminated soil is a promising candidate for microbial remediation of Cd²⁺ contamination.

Keywords: Antibiotic resistance; Cadmium; Enterobacter hormaechei SFC3; Metal tolerance; Remediation.

MeSH terms

Cadmium* / toxicity
Enterobacter / genetics
RNA, Ribosomal, 16S
Soil
Soil Pollutants* / toxicity

Substances

Cadmium
Soil
potassium nitrate
RNA, Ribosomal, 16S
Soil Pollutants