3D diversiform dynamic process of microvilli in living cells

Yuhui Wei; Kaizhe Wang; Qinglin Xia; Bin Li; Lin Liu

doi:10.1016/j.bbrc.2022.10.002

3D diversiform dynamic process of microvilli in living cells

Biochem Biophys Res Commun. 2022 Dec 20:635:114-119. doi: 10.1016/j.bbrc.2022.10.002. Epub 2022 Oct 6.

Authors

Yuhui Wei¹, Kaizhe Wang², Qinglin Xia¹, Bin Li³, Lin Liu⁴

Affiliations

¹ Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 201800, China; The Interdisciplinary Research Center, Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210, China.
² Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology & Engineering, Chinese Academy of Sciences (CAS), Ningbo, 315300, PR China.
³ Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 201800, China; The Interdisciplinary Research Center, Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210, China. Electronic address: libin@sinap.ac.cn.
⁴ Key Laboratory of Laboratory Medicine, Ministry of Education of China, Zhejiang Provincial Key Laboratory of Medical Genetics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, 325035, China. Electronic address: liulin0829@wmu.edu.cn.

PMID: 36265284
DOI: 10.1016/j.bbrc.2022.10.002

Abstract

Microvilli are membrane protrusions involved in many membrane-associated physiological processes. Previous studies have focused on the dynamics of individual microvilli, however, the morphological classification of microvilli and the dynamics of microvillar clusters as the basic functional domain remain largely unknown. Here we used atomic force microscopy (AFM) to achieve nanoscale resolution 3D microvilli images of living HeLa cells. We found that there were mainly two subtypes of microvilli with different morphologies and lifecycle that were unequally present on the cell membrane. Employing a strategy to track microvillar cluster movement at nanometer resolution, we further revealed the polymorphic movement of microvillar clusters in 3D. Overall, these data strengthened the morphology and dynamics of cell membranes and associated structures, which provided a new perspective for microvillar function research.

Keywords: Atomic force microscopy; Dynamic; Microvilli.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Membrane / metabolism
Endocytosis*
HeLa Cells
Humans
Microscopy, Atomic Force / methods
Microvilli / metabolism