A plant wide simulation of polyhydroxyalkanoate production from wastewater and its conversion to methyl crotonate

Abstract

This work simulates the production of methyl crotonate from various industrial wastewaters. In the upstream process, wastewater is fermented into volatile fatty acids which are then converted into polyhydroxyalkanoates (PHA) by means of mixed microbial cultures. In the downstream, PHA undergoes a series of thermolysis and esterification reactions to produce methyl crotonate. The origin of the wastewater was found to have a great influence on the composition of the PHA with the effluent of a candy bar factory producing a high polyhydroxybutyrate/polyhydroxyvalerate ratio of 86/14 in favour of methyl crotonate production. It was observed that the use of intracellular polyhydroxybutyrate, instead of purified, significantly lowers the number of separation steps and yet reduces the methyl crotonate recovery by only 20 %. An operating pressure higher than 18 bar led to more transesterification of polyhydroxybutyrate, producing byproducts instead of methyl crotonate. Finally, a 3 h reaction was found sufficient for completion of polyhydroxybutyrate conversion.

Keywords: Biorefinery; Methyl crotonate; Polyhydroxyalkanoate; Process simulation; Waste valorisation.