Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

Dasom Mun; Ji-Young Kang; Yumin Chun; Da-Seul Park; Hyoeun Kim; Nuri Yun; Seung-Hyun Lee; Boyoung Joung

doi:10.1016/j.scr.2022.102940

Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

Stem Cell Res. 2022 Dec:65:102940. doi: 10.1016/j.scr.2022.102940. Epub 2022 Oct 13.

Authors

Dasom Mun¹, Ji-Young Kang¹, Yumin Chun¹, Da-Seul Park¹, Hyoeun Kim², Nuri Yun³, Seung-Hyun Lee², Boyoung Joung⁴

Affiliations

¹ Division of Cardiology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea.
² Department of Biochemistry and Molecular Biology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea.
³ Institute of Life Science & Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea.
⁴ Division of Cardiology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea. Electronic address: cby6908@yuhs.ac.

PMID: 36260954
DOI: 10.1016/j.scr.2022.102940

Abstract

PITX2 is a homeobox gene located in the human 4q25 locus and is commonly associated with atrial fibrillation (AF). Here, we generated two PITX2 knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 genome editing. The edited iPSCs maintained fullpluripotency, normal karyotype and spontaneousdifferentiation capability. This cell line provides a suitable model for investigating the physiopathologyof PITX2 mutation in atrial fibrillation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Atrial Fibrillation*
CRISPR-Cas Systems / genetics
Humans
Induced Pluripotent Stem Cells*