Lysobacter selenitireducens sp. nov., isolated from river sediment

Abstract

A Gram-stain-negative, yellow-pigmented, motile, flagellated and rod-shaped bacterium, designated as 13A^T, was isolated from a river sediment sample of Fuyang River in Hengshui City, Hebei Province, PR China. Strain 13A^T grew at 10-37 °C (optimum, 30 °C), at pH 5.0-11.0 (optimum, pH 7.0) and at 0-7 % (w/v) NaCl concentration (optimum, 0 %). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain 13A^T belongs to the genus Lysobacter, and was most closely related to Lysobacter spongiicola DSM 21749^T (97.8 %), Lysobacter concretionis DSM 16239^T (97.5 %), Lysobacter daejeonensis GIM 1.690^T (97.3 %) and Lysobacter arseniciresistens CGMCC 1.10752^T (96.9 %). Meanwhile, the type species Lysobacter enzymogenes ATCC 29487^T was selected as a reference strain (95.2 %). The genomic size of strain 13A^T was 3.0 Mb and the DNA G+C content was 69.0 %. The average nucleotide identity values between strain 13A^T and each of the reference type strains L. spongiicola DSM 21749^T, L. concretionis DSM 16239^T, L. daejeonensis GIM 1.690^T, L. arseniciresistens CGMCC 1.10752^T and L. enzymogenes ATCC 29487^T were 75.9, 76.1, 77.7, 78.0 and 73.2 %, respectively. The digital DNA-DNA hybridization values between strain 13A^T and each of the reference type strains were 21.7, 22.2, 21.9, 22.7 and 23.2 %, respectively. The average amino acid identity values between strain 13A^T and each of the reference type strains were 72.5, 72.9, 72.3, 75.0 and 69.2 %, respectively. The major fatty acids were iso-C_{15 : 0}, iso-C_{16 : 0} and summed feature 9 (iso-C_{17 : 1} ω9c and/or C_{16 : 0} 10-methyl). The sole respiratory quinone was identified as ubiquinone-8. The polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid, an unidentified lipid, four unidentified phospholipids and two unidentified glycolipids. Based on the phenotypic, physiological, phylogenetic and chemotaxonomic data, strain 13A^T represents a novel species of the genus Lysobacter, for which the name Lysobacter selenitireducens sp. nov. is proposed. The type strain is 13A^T (=JCM 34786^T=GDMCC 1.2722^T).

Keywords: Lysobacter selenitireducens sp. nov.; Venn diagram; genome; phylogenomic tree.