Complete Genome Sequence of Brucella abortus 2308, Isolated from an Abortion Storm on a Dairy Farm in India

Amit Kumar; Malyaj R Prajapati; Surendra Upadhyay; Anamika Bhordia; Vinod Kumar Singh; Jitender Singh; Anu Rahal; Ravindra Kumar; Anil Sirohi

doi:10.1128/mra.00550-22

Complete Genome Sequence of Brucella abortus 2308, Isolated from an Abortion Storm on a Dairy Farm in India

Microbiol Resour Announc. 2022 Nov 17;11(11):e0055022. doi: 10.1128/mra.00550-22. Epub 2022 Oct 19.

Authors

Amit Kumar¹, Malyaj R Prajapati¹, Surendra Upadhyay¹, Anamika Bhordia¹, Vinod Kumar Singh², Jitender Singh¹, Anu Rahal³, Ravindra Kumar¹, Anil Sirohi¹

Affiliations

¹ College of Biotechnology, Sardar Vallbhbhai Patel University of Agriculture & Technology, Meerut, Uttar Pradesh, India.
² College of Veterinary Sciences, UP Pt. Deen Dayal Uphadhayay Pashu Chikitsa Vigyan Viswavidhyalay Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India.
³ Division of Animal Health, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, India.

Abstract

The present report communicates the first complete genome sequence of Brucella abortus strain 2308, isolated from an abortion storm on a dairy farm in India. Bacteria were isolated in pure culture from the placentas of aborted calves, and next-generation sequencing (NGS) revealed that the genome sequence length of the isolated strain is 3,285,606 bp, with a GC content of 57.25%, an N₅₀ value of 296,426 bp, and an L₅₀ value of 4, containing 3,119 coding DNA sequences (CDSs), 49 tRNAs, 1 transfer-messenger RNA (tmRNA), and 3 rRNA genes.