We demonstrate optical spectrum detection of a synthetic silica microsphere (SSM) resonator with whispering gallery modes fabricated by chemical methods using an optical nanofiber to touch the SSM. Critical coupling, under coupling and over coupling are obtained by controlling the nanofiber radius. The SSM radius deviation, 0.51 nm, can be obtained through multiple measurements when the nanofiber touches the SSM equatorial planes randomly. The scheme opens a new avenue for accurate sample characterization and sample tracking for microparticle detection.