A biocompatible, reliable and quick responsive fiber-optic sensor based on Mach-Zehnder interferometer (MZI) is demonstrated for nitrate analytes tracing. The sensor was constructed by collapsing the air holes of a short length photonic crystal fiber (PCF) with the single-mode fibers (SMFs) on both ways. The proposed sensor has been coated with a graphene-PVA (polyvinyl alcohol) membrane using the thermal coating technique to make the sensor attractive to the nitrate ions in the aqueous solution. The maximum response is found to be 0.15 pm/ppm on the nitrate measurement scale of 0 ppm to 100 ppm with an average reaction time of ∼10 s. Also, a short length of FBG (fiber Bragg grating) is implanted with SMF to improve the sensing accuracy of the presented sensor.