Paper-based colorimetric glucose sensor using Prussian blue nanoparticles as mimic peroxidase

Liu Tong; Lina Wu; Yunfeng Zai; Yu Zhang; Enben Su; Ning Gu

doi:10.1016/j.bios.2022.114787

Paper-based colorimetric glucose sensor using Prussian blue nanoparticles as mimic peroxidase

Biosens Bioelectron. 2023 Jan 1:219:114787. doi: 10.1016/j.bios.2022.114787. Epub 2022 Oct 6.

Authors

Liu Tong¹, Lina Wu², Yunfeng Zai², Yu Zhang³, Enben Su⁴, Ning Gu⁵

Affiliations

¹ State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210009, PR China; Getein Biotechnology Co., Ltd, Nanjing, 210000, China.
² Getein Biotechnology Co., Ltd, Nanjing, 210000, China.
³ State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210009, PR China.
⁴ Getein Biotechnology Co., Ltd, Nanjing, 210000, China. Electronic address: enbens@njmu.edu.cn.
⁵ State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210009, PR China. Electronic address: guning@seu.edu.cn.

PMID: 36257117
DOI: 10.1016/j.bios.2022.114787

Abstract

A novel paper-based colorimetric glucose sensor was proposed employing Prussian blue nanoparticles (PB NPs) as mimic peroxidase. The sensor was manufactured by spraying solution containing PB NPs, glucose oxidase and chromogenic agents into a paper, then coating the filter layer and spreading layer on the top. The layer-by-layer structure enabled the sensor detect glucose in whole blood, as well as elimination of the coffee-ring effect which ensure the performance. As a powerful alternative to natural peroxidase, PB NPs showed the mimic enzymatic activity well preserved in dry environment. The manufacture process of the sensor is easy to be industrialized. Under optimal conditions, the sensor exhibited a linear range from 2.5 mM to 25 mM for glucose in blood with satisfactory reproducibility (the coefficient of variant <4%), great storage stability (1 month at 45 °C) and excellent linearity compared with those commercial kits (R > 0.99). Coupled with a handhold device, the PB NPs-based test strip realized the goal of personal operation, user-friendly control, automatic readouts, and data storage, and able to link the Cloud, showing unique potential in clinical application, especially in community-level medical scenarios.

Keywords: Glucose; Handheld system; Paper-based sensor; Prussian blue; Whole blood.