Monitoring Changes in Intracellular Reactive Oxygen Species Levels in Response to Histone Deacetylase Inhibitors

Al-Hassan M Mustafa; Ramy Ashry; Oliver H Krämer

doi:10.1007/978-1-0716-2788-4_22

Monitoring Changes in Intracellular Reactive Oxygen Species Levels in Response to Histone Deacetylase Inhibitors

Methods Mol Biol. 2023:2589:337-344. doi: 10.1007/978-1-0716-2788-4_22.

Authors

Al-Hassan M Mustafa^{1

2}, Ramy Ashry^{3

4}, Oliver H Krämer³

Affiliations

¹ Institute of Toxicology, University Medical Center Mainz, Mainz, Germany. alabdeen@uni-mainz.de.
² Department of Zoology, Faculty of Science, Aswan University, Aswan, Egypt. alabdeen@uni-mainz.de.
³ Institute of Toxicology, University Medical Center Mainz, Mainz, Germany.
⁴ Department of Oral Pathology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.

PMID: 36255635
DOI: 10.1007/978-1-0716-2788-4_22

Abstract

Reactive oxygen species (ROS) are induced by several chemotherapeutics. In this protocol, we describe a flow cytometry-based method for the analysis of the intracellular levels of ROS in vital leukemic cells in response to the histone deacetylase inhibitor vorinostat. This measurement of ROS using the cell-permeable dye CM-H2DCFDA indicates intracellular oxidative stress.

Keywords: Flow cytometry; HDACi; Leukemia; ROS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis
Cell Line, Tumor
Histone Deacetylase Inhibitors* / pharmacology
Hydroxamic Acids / pharmacology
Oxidative Stress*
Reactive Oxygen Species
Vorinostat / pharmacology

Substances

Histone Deacetylase Inhibitors
Reactive Oxygen Species
Vorinostat
Hydroxamic Acids