An ultra-sensitive photothermal lateral flow immunoassay for 17β-estradiol in food samples

Yi Zhang; Han-Jie Yang; Zhenghua Xu; Xiao Liu; Jie Zhou; Xu-Fang Qu; Wen-Long Wang; Yongwei Feng; Chifang Peng

doi:10.1016/j.foodchem.2022.134482

An ultra-sensitive photothermal lateral flow immunoassay for 17β-estradiol in food samples

Food Chem. 2023 Mar 15;404(Pt A):134482. doi: 10.1016/j.foodchem.2022.134482. Epub 2022 Oct 1.

Authors

Yi Zhang¹, Han-Jie Yang², Zhenghua Xu³, Xiao Liu², Jie Zhou², Xu-Fang Qu², Wen-Long Wang², Yongwei Feng⁴, Chifang Peng²

Affiliations

¹ State Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China. Electronic address: zhangyijnu@jiangnan.edu.cn.
² State Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
³ Huangpu Customs Technology Center, Guangzhou 510770, China.
⁴ Technology Innovation Center of Special Food for State Market Regulation, Wuxi Food Safety Inspection and Test Center, Wuxi 214100, China.

PMID: 36252380
DOI: 10.1016/j.foodchem.2022.134482

Abstract

Hormone residues in food and drinking water endanger human health, therefore, on-site analysis techniques of superior performance are important for monitoring this risk. In this study, an ultra-sensitive photothermal lateral flow immunoassay (LFIA) for quantification of 17β-estradiol (E₂) has been developed. Anti-E₂ antibody modified black phosphorus-Au (BP-Au) nanocomposite was developed as a photothermal contrast signal probe and the temperature at test-zone was recorded with an infrared camera. Under the irradiation of 808 nm laser at test-zone, it gave temperatures negatively related to the concentrations of E₂ in samples. Under optimal detecting conditions, the developed photothermal LFIA exhibited a limit of detection of 50 pg mL^-1, over 100-fold more sensitive than visual LFIA, and a linear range of 3 orders of magnitude. This method has been successfully applied to water, milk, and milk powder samples.

Keywords: 17β-estradiol; Black phosphorus; Lateral flow immunoassay; Photothermal.

MeSH terms

Animals
Antibodies
Estradiol* / analysis
Gold / chemistry
Humans
Immunoassay / methods
Limit of Detection
Milk* / chemistry
Phosphorus / analysis

Substances

Estradiol
Phosphorus
Antibodies
Gold