Investigating Antimicrobial Resistance and ESBL Producing Gene in Klebsiella Isolates among Neonates and Adolescents in Southern Bangladesh

Afroza Akter Tanni; Nahid Sultana; Wazir Ahmed; Md Mahbub Hasan; Md Shakhawat Hossain; Sajjad Hossain Noyon; Md Mobarok Hossain; Adnan Mannan

doi:10.1155/2022/7071009

Investigating Antimicrobial Resistance and ESBL Producing Gene in Klebsiella Isolates among Neonates and Adolescents in Southern Bangladesh

Can J Infect Dis Med Microbiol. 2022 Sep 30:2022:7071009. doi: 10.1155/2022/7071009. eCollection 2022.

Authors

Afroza Akter Tanni^{1

2}, Nahid Sultana³, Wazir Ahmed⁴, Md Mahbub Hasan^{1

2}, Md Shakhawat Hossain^{1

2}, Sajjad Hossain Noyon^{1

2}, Md Mobarok Hossain¹, Adnan Mannan^{1

2}

Affiliations

¹ Department of Genetic Engineering & Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chattogram-4331, Bangladesh.
² Disease Biology and Molecular Epidemiology Research Lab, Biotechnology Research & Innovation Centre (BRIC), Department of Genetic Engineering & Biotechnology, University of Chittagong, Chattogram, Bangladesh.
³ Department of Microbiology, Chattogram Maa O Shishu Hospital, Agrabad, Chattogram, Bangladesh.
⁴ Department of Neonatology, Chattogram Maa O Shishu Hospital, Agrabad, Chattogram, Bangladesh.

Abstract

Background: Multidrug-resistant (MDR) clones of Klebsiella pneumoniae (Kpn) have been increasingly documented in community-acquired and nosocomial infections all around the globe. Extended-spectrum β-lactamases (ESBLs) are a rapidly evolving group of β-lactamase enzymes derived from SHV genes by mutations. This research work aimed to investigate and analyze the widespread prevalence of Kpn antibiotic resistance in different areas of the southern part of Bangladesh.

Methods: This particular study was executed and implemented by using 501 clinical samples or isolates from two different hospitals in Chattogram. The disk diffusion method was used to detect Kpn's sensitivity to 16 antibiotics in a drug susceptibility test. By using the PCR technique, the widespread prevalence of antibiotic-resistant gene bla_SHV-11 was studied. Sequencing along with phylogenetic analysis was utilized to verify isolates with the bla_SHV-11 gene.

Results: Almost all of the Kpn isolates were spotted to be antibiotic-resistant. These Kpn isolates were resistant to β-lactams, aminoglycosides, and quinolones at high levels. The spatial analysis displayed that infections involving Kpn were more common in the urban areas (70%) than in the rural areas (30%). Neonates had substantially higher levels (p < 0.001) of resistance to multidrug than other age groups. Cefepime was identified as the most frequent antibiotic-resistant to all age groups (56.68%). The highest numbers of resistant isolates (36.92%) were found in urine samples. The ESBL gene bla_SHV-11 was found in 38% isolates.

Conclusion: The significant frequency of MDR Kpn harboring β-lactamases and AMR genes strongly suggests the requirement to develop effective antimicrobial resistance control and prevention measures in Bangladesh.