Expression of SR-BI and LXR α in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide

Contrast Media Mol Imaging. 2022 Sep 27:2022:3920584. doi: 10.1155/2022/3920584. eCollection 2022.

Abstract

Objective: To investigate the influences of Astragalus polysaccharides (APS) on the expressions of SR-BI(Scavenger receptor-BI) and LXR α in RAW264.7 macrophage origin foam cells.

Methods: Mouse RAW264.7 cells were induced in foam cells and identification of the foamed by oil-red O staining. The RAW264.7 macrophage origin foam cells were dealt with APS with distinct contents (0, 10, 20, 50, or 100 mg/L). The mRNA and protein expressions of SR-BI and LXRα were measured by RT-PCR as well as ELISA, respectively.

Results: Macrophages were differentiated into foam cells 48 h after ox-LDL induction. In contrast to the control part, the mRNA and protein expressions of SR-BI and LXRα in RAW264.7 macrophage origin foam cells were up-regulated dosage-dependently after being treated with different concentrations of APS (P < 0.05).

Conclusion: APS could promote intracellular cholesterol efflux by up-regulating the expressions of SR-BI and LXR α of RAW264.7 cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Retracted Publication

MeSH terms

  • Animals
  • Atherosclerosis* / metabolism
  • Cell Line
  • Cholesterol / metabolism
  • Foam Cells* / metabolism
  • Liver X Receptors / metabolism*
  • Macrophages / metabolism
  • Mice
  • Polysaccharides / metabolism
  • Polysaccharides / pharmacology
  • RNA, Messenger / metabolism
  • Receptors, Scavenger / metabolism
  • Scavenger Receptors, Class B / metabolism*

Substances

  • Liver X Receptors
  • Polysaccharides
  • RNA, Messenger
  • Receptors, Scavenger
  • Scarb1 protein, mouse
  • Scavenger Receptors, Class B
  • Cholesterol