A Comparative Study of Voltammetric vs Impedimetric Immunosensor for Rapid SARS-CoV-2 Detection at the Point-of-care

Cristina Tortolini; Antonio Angeloni; Riccarda Antiochia

doi:10.1002/elan.202200349

A Comparative Study of Voltammetric vs Impedimetric Immunosensor for Rapid SARS-CoV-2 Detection at the Point-of-care

Electroanalysis. 2022 Sep 29:10.1002/elan.202200349. doi: 10.1002/elan.202200349. Online ahead of print.

Authors

Cristina Tortolini¹, Antonio Angeloni¹, Riccarda Antiochia²

Affiliations

¹ Department of Experimental Medicine University of Rome "La Sapienza" Viale Regina Elena 324 00166 Rome Italy.
² Department of Chemistry and Drug Technologies University of Rome "La Sapienza" P.le Aldo Moro 5 00185 Rome Italy.

Abstract

Here, a novel biosensing platform for the detection of SARS-CoV-2 usable both at voltammetric and impedimetric mode is reported. The platform was constructed on a multi-walled carbon nanotubes (MWCNTs) screen-printed electrode (SPE) functionalized by methylene blue (MB), antibodies against SARS-CoV-2 spike protein (SP), a bioactive layer of chitosan (CS) and protein A (PrA). The voltammetric sensor showed superior performances both in phosphate buffer solution (PBS) and spiked-saliva samples, with LOD values of 5.0±0.1 and 30±2.1 ng/mL, compared to 20±1.8 and 50±2.5 ng/mL for the impedimetric sensor. Moreover, the voltammetric immunosensor was tested in real saliva, showing promising results.

Keywords: Immunosensor; POC diagnostics; SARS-CoV-2; impedimetric biosensor; voltammetric biosensor.