Clonal diversity of Acinetobacter clinical isolates producing NDM-type carbapenemase in Cuba, 2013-19

Dianelys Quiñones Pérez; Meiji Soe Aung; Yenisel Carmona Cartaya; María Karla González Molina; Niurka Pereda Novales; Nobumichi Kobayashi

doi:10.1016/j.ijregi.2022.08.008

Clonal diversity of Acinetobacter clinical isolates producing NDM-type carbapenemase in Cuba, 2013-19

IJID Reg. 2022 Aug 29:5:93-96. doi: 10.1016/j.ijregi.2022.08.008. eCollection 2022 Dec.

Authors

Dianelys Quiñones Pérez¹, Meiji Soe Aung², Yenisel Carmona Cartaya¹, María Karla González Molina¹, Niurka Pereda Novales¹, Nobumichi Kobayashi²

Affiliations

¹ Healthcare-Associated Infections National Laboratory, Pedro Kourí Institute of Tropical Medicine, Havana 11400, Cuba.
² Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan.

Abstract

Objectives: Our study aimed to elucidate the clonal diversity of carbapenem-resistant Acinetobacter clinical isolates producing NDM-type carbapenemase collected through national surveillance in Cuba during a 7-year period (2013-19).

Methods: A total of 199 isolates of Acinetobacter spp. from 37 hospitals in 12 provinces were genetically analyzed for their species, carbapenemase genes and genotypes. Sequence type (ST) and OXA-51-like gene type were determined for bla _NDM-positive isolates.

Results: Most isolates (95%) were identified as species of Acinetobacter calcoaceticus-baumannii complex, with A. baumannii being the majority. Acquired carbapenemase genes were assigned to bla _OXA or bla _NDM type; the most commonly detected gene was bla _OXA-23-like (49%), followed by bla _OXA-24-like (20%) and bla _NDM (15%). Twenty-nine bla _NDM-positive isolates (22 A. baumannii, 2 A. pittii, 2 A. johnsonii, 3 other species) were differentiated into 19 STs, including the most common, ST23. Though NDM genes were mostly typed as bla _NDM-1, a novel bla _NDM-42 was identified in an ST79 A. baumannii isolate. bla _OXA-51-like genes of NDM-positive A. baumannii were discriminated into 10 OXA types, including 2 novel ones.

Conclusions: Our study indicated the spread of bla _NDM to various clones of A. baumannii and other Acinetobacter spp. in Cuba.

Keywords: Acb complex, Acinetobacter calcoaceticus-baumannii complex; Acinetobacter; CRA, carbapenem-resistant Acinetobacter; CRAb, carbapenem-resistant Acinetobacter baumannii; Carbapenem resistance; Cuba; MICs, minimum inhibitory concentrations; NDM; OXA; ST, sequence type; Sequence type.