Molecular detection and identification of three intracellular parasites of retail mutton products in Beijing, China

Zifu Zhu; Yajie Chen; Xu Yang; Lifang Wang; Qun Liu; Jing Liu

doi:10.3389/fvets.2022.1018788

Molecular detection and identification of three intracellular parasites of retail mutton products in Beijing, China

Front Vet Sci. 2022 Sep 30:9:1018788. doi: 10.3389/fvets.2022.1018788. eCollection 2022.

Authors

Zifu Zhu¹, Yajie Chen¹, Xu Yang¹, Lifang Wang¹, Qun Liu^{1

2}, Jing Liu^{1

2}

Affiliations

¹ National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China.
² Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.

Abstract

Sarcocystis spp., Neospora caninum and Toxoplasma gondii are globally ubiquitous pathogens, and domestic sheep are considered to be one of the intermediate hosts. 83 myocardial samples of sheep were collected from 12 retail stores in Beijing, China. Sarcocystis spp., N. caninum and T. gondii were identified by PCR amplification of the 18S rRNA gene, Nc-5 gene and 529bp DNA fragment with a prevalence of 86.7% (95% CI: 77.5-93.2) and 43.4% (95% CI: 32.5-54.7) for Sarcocystis spp. and N. caninum infections, respectively, and no T. gondii was detected. The co-infection prevalence of Sarcocystis and N. caninum was 38.6% (95% CI: 28.1-49.9). Two Sarcocystis species were subtyped by analyzing 18SrRNA sequences and were identified as Sarcocystis tenella and Sarcocystis arieticanis. The prevalence of S. tenella and S. arieticanis infections was 84.3% (95% CI: 74.7-91.4) and 56.6% (95% CI: 45.3-67.5), respectively. This study shows that sheep have a high risk of infection with Sarcocystis and N. caninum, suggests that effective prevention measures are needed to avoid the spread of these parasites in sheep. Toxoplasmosis in sheep poses a threat to human and animal health and requires monitoring and preventing continuously.

Keywords: N. caninum; Sarcocystis spp.; T. gondii; prevalence; sheep.