Complete chloroplast genome of Ligularia biceps Kitam, 1941 (Asteraceae)

Yue-Yue Song; Chang-Neng Shi; Che Bian; Liang Xu; Yan-Yun Yang; Ming Xie; Ting-Guo Kang

doi:10.1080/23802359.2022.2130716

Complete chloroplast genome of Ligularia biceps Kitam, 1941 (Asteraceae)

Mitochondrial DNA B Resour. 2022 Oct 12;7(10):1792-1793. doi: 10.1080/23802359.2022.2130716. eCollection 2022.

Authors

Yue-Yue Song¹, Chang-Neng Shi¹, Che Bian¹, Liang Xu¹, Yan-Yun Yang¹, Ming Xie¹, Ting-Guo Kang¹

Affiliation

¹ School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.

Abstract

Ligularia biceps is a plant belonging to Ligularia Cass., most of which have certain medicinal value. In this study, the chloroplast (cp) genome of L. biceps was sequenced for the first time. The L. biceps cp genome sequence length was 151,153 bp, with an large single-copy (LSC) region length of 83,259 bp, an small single-copy (SSC) region length of 18,234 bp, a pair of inverted repeat regions (IRs) length of 24,830 bp and GC content of 37.5%. In total, 131 genes were annotated, including 86 protein-coding genes, eight rRNA genes, and 37 tRNA genes. The phylogenetic tree was built based on 23 species, using the maximum-likelihood method. The results showed that the species clustered with other Ligularia Cass. species. This study provides a theoretical basis for establishing a classification system.

Keywords: Asteraceae; Ligularia biceps; chloroplast genome; phylogenetic tree.

Grants and funding

This research was funded by 2019 Liaoning Provincial Department of Education Scientific Research Project, China [No. L201942], National Key Research and Development in the 13th Five-Year Plan [No. 2018YFC1708200], Major Special Fund for Science and Technology of Inner Mongolia Autonomous Region [No. 2019ZD004], Natural Science Fund Project of Liaoning Province [No. 2020-MS-224], Liaoning BaiQianWan Talents Program [No. 2021A039], the National Natural Science Foundation of China [General Program, Grant Number 81874338], and Re-Excavation and DNA Molecular Identification of Genuine Medicinal Herbs in Liaoning Province [No. 202110162007].