An ultrasensitive sensing platform based on fluorescence carbon dots for chlorogenic acid determination in food samples

Qin Hu; Wenhui Wang; Zhen-Quan Yang; Lixia Xiao; Xiaojuan Gong; Lizhen Liu; Jie Han

doi:10.1016/j.foodchem.2022.134395

An ultrasensitive sensing platform based on fluorescence carbon dots for chlorogenic acid determination in food samples

Food Chem. 2023 Mar 15;404(Pt A):134395. doi: 10.1016/j.foodchem.2022.134395. Epub 2022 Sep 26.

Authors

Qin Hu¹, Wenhui Wang², Zhen-Quan Yang², Lixia Xiao², Xiaojuan Gong³, Lizhen Liu⁴, Jie Han⁵

Affiliations

¹ College of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225001, PR China. Electronic address: qinhu@yzu.edu.cn.
² College of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225001, PR China.
³ Institute of Environmental Science, and School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China.
⁴ School of Chemistry and Environmental Engineering, Shanxi Datong University, Datong 037009, PR China.
⁵ School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu, PR China. Electronic address: hanjie@yzu.edu.cn.

PMID: 36244071
DOI: 10.1016/j.foodchem.2022.134395

Abstract

In this work, an ultrasensitive and convenient method was established for chlorogenic acid (CGA) determination based on fluorescence quenching of carbon dots (CDs). The CDs were prepared by hydrothermal heating of citric acid and p-phenylenediamine. The proposed CDs-based sensing platform showed high selectivity and sensitivity towards CGA detection. Under optimal working conditions, the fluorescence signals of CDs decreased with increasing of CGA contents and presented linear response to CGA content in two ranges of 0.01-0.1 and 0.1-20.0 μM. The detection limits were as low as 8.87 nM and 0.12 μM. The proposed method was successfully applied to analyze CGA in real food samples. The recoveries were between 98.9 % and 106.7 % and the relative standard deviations (RSDs) were below 3.28 %. This work highlights the construction of a facial, simple, economic and highly sensitive fluorescence sensing system for CGA detection with promising application prospects in food analysis.

Keywords: CDs; CGA; Fluorescence quenching; Food samples.

MeSH terms

Carbon*
Chlorogenic Acid
Fluorescent Dyes
Quantum Dots*
Spectrometry, Fluorescence / methods

Substances

Carbon
Chlorogenic Acid
Fluorescent Dyes