Development of a certified reference material for the accurate determination of type B trichothecenes in corn

Mohamed A Gab-Allah; Yared Getachew Lijalem; Hyeonwoo Yu; Sunyoung Lee; Song-Yee Baek; Jeesoo Han; Kihwan Choi; Byungjoo Kim

doi:10.1016/j.foodchem.2022.134542

Development of a certified reference material for the accurate determination of type B trichothecenes in corn

Food Chem. 2023 Mar 15;404(Pt A):134542. doi: 10.1016/j.foodchem.2022.134542. Epub 2022 Oct 8.

Authors

Mohamed A Gab-Allah¹, Yared Getachew Lijalem², Hyeonwoo Yu³, Sunyoung Lee⁴, Song-Yee Baek⁴, Jeesoo Han⁴, Kihwan Choi⁵, Byungjoo Kim⁶

Affiliations

¹ Organic Metrology Group, Division of Chemical and Biological Metrology, Korea Research Institute of Standards and Science, Daejeon 34113, South Korea; Department of Bio-Analytical Science, University of Science and Technology, Daejeon 34113, South Korea; Reference Materials Lab, National Institute of Standards, Tersa St, Haram, P. O. Box: 136, Giza 12211, Egypt.
² Organic Metrology Group, Division of Chemical and Biological Metrology, Korea Research Institute of Standards and Science, Daejeon 34113, South Korea; Department of Bio-Analytical Science, University of Science and Technology, Daejeon 34113, South Korea; National Metrology Institute of Ethiopia, Addis Ababa, P. O. Box: 5722, Ethiopia.
³ Organic Metrology Group, Division of Chemical and Biological Metrology, Korea Research Institute of Standards and Science, Daejeon 34113, South Korea; Department of Bio-Analytical Science, University of Science and Technology, Daejeon 34113, South Korea.
⁴ Organic Metrology Group, Division of Chemical and Biological Metrology, Korea Research Institute of Standards and Science, Daejeon 34113, South Korea.
⁵ Organic Metrology Group, Division of Chemical and Biological Metrology, Korea Research Institute of Standards and Science, Daejeon 34113, South Korea; Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 34134, South Korea. Electronic address: kihwan.choi@kriss.re.kr.
⁶ Organic Metrology Group, Division of Chemical and Biological Metrology, Korea Research Institute of Standards and Science, Daejeon 34113, South Korea; Department of Bio-Analytical Science, University of Science and Technology, Daejeon 34113, South Korea. Electronic address: byungjoo@kriss.re.kr.

PMID: 36244066
DOI: 10.1016/j.foodchem.2022.134542

Abstract

A corn flour certified reference material (KRISS CRM 108-01-011) was developed to ensure accurate and reliable measurements of type B trichothecenes (deoxynivalenol, nivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol), and deoxynivalenol-3-glucoside. The material was freeze-dried, ground, sieved, and well-mixed. The final produced CRM was packaged at 14 g per unit and stored at -70 °C. The certification was performed using isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS). For simultaneous characterization and homogeneity assessments, ten units were randomly selected and analyzed. The among-unit relative standard deviations were below 1 % for all mycotoxins, indicating excellent homogeneity of the CRM. The stability of the CRM was also assessed at various designated temperatures and test periods. The uncertainties of the certified values varied between 2.4 % and 6.2 %, thereby confirming their higher-order metrological quality to provide references for testing laboratories. In case of deoxynivalenol-3-glucoside, an information value was assigned due to the lack of its traceability to the SI units.

Keywords: Certified reference material; Corn; Homogeneity and stability assessment; Isotope dilution–mass spectrometry; Type B trichothecenes.

MeSH terms

Certification
Chromatography, Liquid / methods
Reference Standards
Tandem Mass Spectrometry / methods
Trichothecenes, Type B*
Zea mays* / chemistry

Substances

deoxynivalenol-3-glucoside
Trichothecenes, Type B