Optimisation and characterization of α-D-glucan produced by Bacillus velezensis RSDM1 and evaluation of its protective effect on oxidative stress in Tetrahymena thermophila induced by H2O2

Reda Derdak; Souraya Sakoui; Oana Lelia Pop; Dan Cristian Vodnar; Boutaina Addoum; Bernadette-Emoke Teleky; Simon Elemer; Abdelhakim Elmakssoudi; Ramona Suharoschi; Abdelaziz Soukri; Bouchra El Khalfi

doi:10.1016/j.ijbiomac.2022.10.095

Optimisation and characterization of α-D-glucan produced by Bacillus velezensis RSDM1 and evaluation of its protective effect on oxidative stress in Tetrahymena thermophila induced by H₂O₂

Int J Biol Macromol. 2022 Dec 1;222(Pt B):3229-3242. doi: 10.1016/j.ijbiomac.2022.10.095. Epub 2022 Oct 13.

Affiliations

¹ Laboratory of Physiopathology, Molecular Genetics & Biotechnology, Faculty of Sciences Ain Chock, Health and Biotechnology Research Centre, Hassan II University of Casablanca, Maarif B.P 5366, Casablanca, Morocco.
² Department of Food Science, University of Agricultural Science and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania; Molecular Nutrition and Proteomics Lab, CDS3, Life Science Institute, University of Agricultural Science and Veterinary Medicine, Calea Mănăștur 3-5, 400372, Cluj-Napoca, Romania.
³ Department of Food Science, University of Agricultural Science and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania; Food Biotechnology and Molecular Gastronomy, CDS7, Life Science Institute, University of Agricultural Science and Veterinary Medicine, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania.
⁴ Department of Food Science, University of Agricultural Science and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania.
⁵ Department of Chemistry, Laboratory of Organic Synthesis, Extraction, and Valorization, Faculty of Sciences Aïn Chock, Hassan II University of Casablanca, Maarif B.P 5366, Casablanca, Morocco.
⁶ Laboratory of Physiopathology, Molecular Genetics & Biotechnology, Faculty of Sciences Ain Chock, Health and Biotechnology Research Centre, Hassan II University of Casablanca, Maarif B.P 5366, Casablanca, Morocco. Electronic address: bouchra.elkhalfi@gmail.com.

PMID: 36243160
DOI: 10.1016/j.ijbiomac.2022.10.095

Abstract

A novel exopolysaccharide was isolated from Bacillus velezensis RSDM1, purified and characterized. The optimization was carried out by Box-Behnken design and the highest yield of EPS-RSDM1 was 1969.53 mg/L. The EPS was composed by glucose with a molecular weight of 1.78 × 10⁴ Da and connected by α-1,6 and α-1,3. The EPS was thermally stable and showed pseudoplastic rheology. The SEM have demonstrated a porous structure. Furthermore, EPS-RSDM1 showed a protective effect against the oxidative stress induced by H₂O₂ in Tetrahymena thermophila. The exposure of the cells to H₂O₂ reduced significantly the cell survival and CAT, SOD, GR, SDH and GAPDH activities, and increased the MDA production. However, pretreatment of the cells with EPS-RSDM1 prior to H₂O₂ exposure elevated the cell survival and enzyme activities, and decreased the level of MDA activity. Our study suggest that EPS-RSDM1 could be useful for preventing cellular oxidation in pharmaceutical and food industries.

Keywords: Bacillus velezensis; Oxidative stress; Structure characterization.

MeSH terms

Glucans*
Hydrogen Peroxide / pharmacology
Molecular Weight
Oxidative Stress
Polysaccharides, Bacterial / chemistry
Tetrahymena thermophila*

Substances

Glucans
Hydrogen Peroxide
Polysaccharides, Bacterial

Supplementary concepts

Bacillus velezensis