This study comprehensively evaluates the ecotoxicity of high-concentration heavy petroleum (HCHP)-contaminated soil before and after thermal desorption (TD) remediation at different temperatures and times. The results showed that the detoxification of contaminated soil was effectively achieved by extending the remediation duration at 400-600 °C. After treatment at 400 °C for 60 min, the toxicological indicators including bioluminescence EC50 (acute toxicity), seed germination ratio (Gr) and plant biomass of Brassica juncea (subacute toxicity), and diversity of the microbial community (chronic toxicity) reached a maximum. The value of the SOS-Induction Factor (SOSIF), characterizing genotoxicity was below 1.5, indicating that it was non-toxic. Pearson's correlation analysis illustrated that the water-soluble fraction (WSF), ALK1-3 and ARO1-3 of petroleum hydrocarbons were the primary sources of ecotoxicity. Notably, although the total ratio of petroleum removed from the soil reached 87.26 ± 4.38 %-98.69 ± 1.61 % under high-temperature thermal desorption (HTTD, 500-600 °C), the ecotoxicity was not lower than that at 400 °C. The pyrolysis products of petroleum macromolecules and extreme changes in soil properties were the leading causes of soil ecotoxicity following HTTD. The inconsistency between the removal of petroleum pollutants and ecological health risks reveals the significance of soil ecotoxicological assessments for identifying TD remediation endpoints and process optimization.
Keywords: Bioluminescence characteristics; Genotoxicity; High-concentration heavy petroleum; Microbial community; Phytotoxicity.
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