Physiological and Transcriptomic Analyses Reveal the Mechanisms of Compensatory Growth Ability for Early Rice after Low Temperature and Weak Light Stress

Hui Wang; Lei Zhong; Xiaoquan Fu; Shiying Huang; Haihui Fu; Xiang Shi; Lifang Hu; Yicong Cai; Haohua He; Xiaorong Chen

doi:10.3390/plants11192523

Physiological and Transcriptomic Analyses Reveal the Mechanisms of Compensatory Growth Ability for Early Rice after Low Temperature and Weak Light Stress

Plants (Basel). 2022 Sep 26;11(19):2523. doi: 10.3390/plants11192523.

Authors

Hui Wang^{1

2

3}, Lei Zhong^{1

2

3}, Xiaoquan Fu^{1

2

3}, Shiying Huang^{1

2

3}, Haihui Fu^{1

2

3}, Xiang Shi^{1

2

3}, Lifang Hu^{1

2

3}, Yicong Cai^{1

2

3}, Haohua He^{1

2

3}, Xiaorong Chen^{1

2

3}

Affiliations

¹ Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China.
² Jiangxi Super Rice Engineering Technology Center, Jiangxi Agricultural University, Nanchang 330045, China.
³ College of Agriculture, Jiangxi Agricultural University, Nanchang 330045, China.

Abstract

"Late spring coldness" (T) is a frequent meteorological disaster in the spring in southern China, often causing severe yield losses of direct-seeded early rice. In this study, we investigated the mechanisms underlying the differences in the compensatory growth ability of different rice genotypes by focusing on agronomic traits, physiological indicators, and transcriptome. The results showed that there were significant differences in the compensatory growth recovery ability of different genotypes after a combination of four days of low temperature and weak light stress. Only the strong compensatory growth genotype B116 was able to grow rapidly and reduce soluble protein and H₂O₂ concentrations rapidly after stress. By analyzing enzyme activity as well as endogenous hormone concentration, we found that the high superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities and high levels of abscisic acid (ABA) could reduce the damage of B116 during stress. Meanwhile, higher glutamine synthetase (GS) and nitrate reductase (NR) activity and higher levels of gibberellin A₃(GA₃), indoleacetic acid (IAA), and zeatin nucleoside (ZR) could enable B116 to grow rapidly after stress. The identified differentially expressed genes (DEGs) indicated that there were large differences in POD-related genes and gibberellin metabolism between B116 and B144 after stress; RT-PCR quantification also showed a trend consistent with RNA-seq, which may be an important reason for the differences in compensatory growth ability.

Keywords: antioxidant enzymes; combined low temperature and weak light stress; compensatory growth; endogenous hormones; nitrogen metabolizing enzymes; rice genotype; transcriptome.

Abstract

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