A study was conducted to check the efficacy of microbial pathways for calcite precipitation to heal pre-existing cracks in mortar. In this experiment, realistic cracks of varying widths were induced on a mortar sample. Different repair methods were applied to a total of 22 mortar samples. Twelve cracked mortar samples with average crack widths ranging from 0.29 to 1.08 mm were subjected to biodeposition treatment using calcium lactate as a food source. The remaining ten samples were split into two groups: five cracked mortar samples were exclusively exposed to the bacterial solution, while the remaining five samples were immersed in distilled water. Digital image processing was used to extract the crack characteristics before and after the repair application. During several repair cycles, image processing was used to track healing. Further, these repaired mortar samples underwent examination for water permeability, ultra-sonic examination, and examination for recovered compressive strength. A forensic examination of the healing product in terms of morphology and elemental composition was conducted using RAMAN, XRD, SEM-EDS, and TGA. The water permeability of the repaired mortar sample using biodeposition with Ca-lactate was dramatically reduced, but samples treated with bacterial solution and distilled water did not exhibit any significant reduction. Complete crack healing was observed when using Ca-lactate as a food source for microbial repair. The forensic analysis confirmed the presence of calcite in healing precipitates.
Keywords: RAMAN; bacteria; biodeposition treatment; image analysis; microstructure; mortar; permeability; recovered compressive strength; ultra-sonic investigation.