Study on the mechanisms of enhanced biological nitrogen and phosphorus removal by denitrifying phosphorus removal in a Micro-pressure swirl reactor

Yunguang Ma; Weiqing Jiang; Zebing Nie; Peng Qi; Yang Jiao; Jiaxi Peng; Dejun Bian

doi:10.1016/j.biortech.2022.128093

Study on the mechanisms of enhanced biological nitrogen and phosphorus removal by denitrifying phosphorus removal in a Micro-pressure swirl reactor

Bioresour Technol. 2022 Nov:364:128093. doi: 10.1016/j.biortech.2022.128093. Epub 2022 Oct 10.

Authors

Yunguang Ma¹, Weiqing Jiang¹, Zebing Nie², Peng Qi¹, Yang Jiao¹, Jiaxi Peng¹, Dejun Bian³

Affiliations

¹ Changchun Institute of Technology, Changchun 130012, China; Key Laboratory of Urban Sewage Treatment of Jilin Province , Changchun 130012, China.
² Key Laboratory of Urban Sewage Treatment of Jilin Province , Changchun 130012, China; Science and Technology Innovation Center for Municipal Wastewater Treatment and Water Quality Protection, Northeast Normal University, Changchun 130117, China.
³ Changchun Institute of Technology, Changchun 130012, China; Key Laboratory of Urban Sewage Treatment of Jilin Province , Changchun 130012, China. Electronic address: ccgcxybiandj@163.com.

PMID: 36229011
DOI: 10.1016/j.biortech.2022.128093

Abstract

To reveal the mechanisms of enhanced biological nitrogen and phosphorus removal by denitrifying phosphorus removal in a Micro-pressure swirl reactor (MPSR), this study used a MPSR to treat municipal wastewater and enriched denitrifying phosphate accumulating organisms (DPAOs) by using its alternating anaerobic-anoxic-aerobic environment. The coupling of denitrification phosphorus removal (DPR) and simultaneous nitrification endogenous denitrification phosphorus removal (SNEDPR) was achieved in MPSR, and the average removal rates of COD, NH₄⁺-N, TN and TP were 91.57%, 98.51%, 85.88%, 96.08% respectively. The results of the batch experiments showed that DPAOs activity in the low dissolved oxygen (DO) and high DO zones were 70.5% and 74.3%. The results of intracellular carbon source conversion patterns, microbial assays and functional gene prediction indicated that Flavobacterium and Dechloromonas dominated the DPR process in the low DO zone. Based on these findings, nutrient removal pathways within the MPSR were integrated.

Keywords: Denitrifying phosphorus removal; Dissolved oxygen; Metabolic pathway; Micro-pressure swirl reactor; Microbial community structure.