Electrochemiluminescence resonance energy transfer of MnCO3 for ultrasensitive amyloid-β protein detection

Abstract

A composite material MnCO₃/poly(diallyl dimethyl ammonium chloride) (PDDA)/Ag with excellent electrochemiluminescence (ECL) performance and high biocompatibility was prepared by adding MnCO₃ and PDDA to silver nanoparticles (AgNPs). MnCO₃/PDDA/Ag and Au@SiO₂NPs were used as ECL donors and acceptors, respectively. Thus, an effective ECL-resonance energy transfer (RET) sensing platform was established. In a potassium persulfate (K₂S₂O₈) medium, MnCO₃ exhibited ECL emission with an ECL band appearing at 500-600 nm. In addition, Au@SiO₂ nanoparticles showed a UV-visible absorption at 450-650 nm. The ECL emission spectra of MnCO₃ overlapped with the absorption spectra of Au@SiO₂NPs. The effective ECL quenching resulted in a good response to the concentration of Aβ₄₂ in serum samples. The linear range was 5 fg ⋅ mL^-1 to 100 ng ⋅ mL^-1, and the detection limit was 2 fg ⋅ mL^-1. The recovery ranged from 97.7% to 104%. The high-efficiency ECL-RET immunosensor has potential application in detecting human serum Aβ₄₂ and other biomarkers, and can be used for the early screening of diseases.

Keywords: Amyloid-β; Electrochemiluminescence; Gold nanoparticle; Manganese carbonate; Resonance energy transfer.