In this study, an alkaline phosphatase (ALP)-mediated fluorescence immunoassay for detecting zearalenone (ZEN) was established based on the oxVB1 fluorescence signal modulated by MnO2 nanosheets (MnO2 NS). As the ALP-antibody content increased, more 2-phosphoascorbic acid (AAP) was hydrolyzed to ascorbic acid (AA) which destroyed the MnO2 NS rapidly. In the lack of MnO2 NS, VB1 cannot be oxidized to oxVB1 for emitting fluorescence. On the contrary, the fluorescence of oxVB1 recovered slowly with the decrease of the ALP-antibody concentration. In the optimization condition, the detection limit of this method was 15.5 pg mL-1. Moreover, the recovery of ZEN in real samples ranged from 94.24 % to 108.26 %, which indicated the remarkable accuracy and reliability of this approach. Meanwhile, the proposal of this fluorescence immunoassay provided a new possibility for detecting other targets by replacing antibodies and antigens.
Keywords: Alkaline phosphatase; Fluorescence immunoassay; MnO(2) nanosheets; VB(1); Zearalenone.
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