Phase Separation-Based Biochemical Assays for Biomolecular Interactions

Abstract

A variety of protein functions are carried out by protein complexes. Identifying and understanding protein-protein interactions (PPIs) will shed light on the structural foundations of the complexity of life. Although multiple methods have been developed to detect protein-protein interactions (PPIs), few are suited for high-throughput analysis and many of them suffer from severe false-positive and/or false-negative results. Here, we have summarized the previously established methods based on phase separation, namely, CEBIT and CoPIC, for simple, sensitive, and efficient identification of PPIs and further high-throughput screening of PPI regulators in vitro and in vivo, respectively.

Keywords: CEBIT; CoPIC; High-throughput screening; Phase separation; Protein-protein interactions.