Fluorescence Correlation Spectroscopy and Phase Separation

Juan Jeremías Incicco; Debjit Roy; Melissa D Stuchell-Brereton; Andrea Soranno

doi:10.1007/978-1-0716-2663-4_8

Fluorescence Correlation Spectroscopy and Phase Separation

Methods Mol Biol. 2023:2563:161-198. doi: 10.1007/978-1-0716-2663-4_8.

Authors

Juan Jeremías Incicco^{1

2}, Debjit Roy^{1

2}, Melissa D Stuchell-Brereton^{1

2}, Andrea Soranno^{3

4}

Affiliations

¹ Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, St. Louis, MO, USA.
² Center for Science and Engineering of Living Systems (CSELS), Washington University in St Louis, St. Louis, MO, USA.
³ Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, St. Louis, MO, USA. soranno@wustl.edu.
⁴ Center for Science and Engineering of Living Systems (CSELS), Washington University in St Louis, St. Louis, MO, USA. soranno@wustl.edu.

PMID: 36227473
DOI: 10.1007/978-1-0716-2663-4_8

Abstract

A quantitative understanding of the forces controlling the assembly and functioning of biomolecular condensates requires the identification of phase boundaries at which condensates form as well as the determination of tie-lines. Here, we describe in detail how Fluorescence Correlation Spectroscopy (FCS) provides a versatile approach to estimate phase boundaries of single-component and multicomponent solutions as well as insights about the transport properties of the condensate.

Keywords: Biomolecular condensates; Fluorescence Correlation Spectroscopy; Intrinsically disordered proteins; Phase separation.

MeSH terms

Spectrum Analysis*