Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense

Xiong Liu; Zhongliang Yin; Yubo Wang; Sai Cao; Wei Yao; Jinling Liu; Xuedan Lu; Feng Wang; Guilian Zhang; Yunhua Xiao; Wenbang Tang; Huabing Deng

doi:10.3389/fpls.2022.980424

Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense

Front Plant Sci. 2022 Sep 26:13:980424. doi: 10.3389/fpls.2022.980424. eCollection 2022.

Authors

Xiong Liu^{1

2}, Zhongliang Yin^{1

2}, Yubo Wang^{1

2}, Sai Cao^{1

2}, Wei Yao¹, Jinling Liu¹, Xuedan Lu^{1

2}, Feng Wang^{1

2}, Guilian Zhang^{1

2}, Yunhua Xiao^{1

2}, Wenbang Tang^{1

2

3

4}, Huabing Deng^{1

2}

Affiliations

¹ College of Agronomy, Hunan Agricultural University, Changsha, China.
² Hunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, Changsha, China.
³ Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha, China.
⁴ State Key Laboratory of Hybrid Rice, Changsha, China.

Abstract

Plant cell wall is a complex and changeable structure, which is very important for plant growth and development. It is clear that cell wall polysaccharide synthases have critical functions in rice growth and abiotic stress, yet their role in plant response to pathogen invasion is poorly understood. Here, we describe a dwarf and narrowed leaf in Hejiang 19 (dnl19) mutant in rice, which shows multiple growth defects such as reduced plant height, enlarged lamina joint angle, curled leaf morphology, and a decrease in panicle length and seed setting. MutMap analysis, genetic complementation and gene knockout mutant show that cellulose synthase-like D4 (OsCSLD4) is the causal gene for DNL19. Loss function of OsCSLD4 leads to a constitutive activation of defense response in rice. After inoculation with rice blast and bacterial blight, dnl19 displays an enhanced disease resistance. Widely targeted metabolomics analysis reveals that disruption of OsCSLD4 in dnl19 resulted in significant increase of L-valine, L-asparagine, L-histidine, L-alanine, gentisic acid, but significant decrease of L-aspartic acid, malic acid, 6-phosphogluconic acid, glucose 6-phosphate, galactose 1-phosphate, gluconic acid, D-aspartic acid. Collectively, our data reveals the importance of OsCSLD4 in balancing the trade-off between rice growth and defense.

Keywords: cellulose synthase-like D; disease resistance; rice; trade-off; widely targeted metabolomics.