Treatment of Muscle Injury with Stem Cells - Experimental Study in Rabbits

Alex de Lima Santos; Camila Gonzaga da Silva; Leticia Siqueira de Sá Barreto; Marcel Jun Sugawara Tamaoki; Bruno Fiorelini Pereira; Fernando Gonçalves de Almeida; Flavio Faloppa

doi:10.1055/s-0041-1741447

Treatment of Muscle Injury with Stem Cells - Experimental Study in Rabbits

Rev Bras Ortop (Sao Paulo). 2022 Feb 7;57(5):788-794. doi: 10.1055/s-0041-1741447. eCollection 2022 Oct.

Authors

Alex de Lima Santos¹, Camila Gonzaga da Silva², Leticia Siqueira de Sá Barreto², Marcel Jun Sugawara Tamaoki¹, Bruno Fiorelini Pereira³, Fernando Gonçalves de Almeida², Flavio Faloppa¹

Affiliations

¹ Departamento de Ortopedia e Traumatologia da Escola Paulista de Medicina da Universidade Federal de São Paulo, São Paulo, SP, Brasil.
² Departamento de Cirurgia da Escola Paulista de Medicina da Universidade Federal de São Paulo, São Paulo, Brasil.
³ Departamento de Ciências Biológicas, Campus Diadema, UNIFESP, São Paulo, Brasil.

Abstract

Objective Histological and macroscopic evaluation of the healing process of acute lesions of the femoral rectus muscle using stem cells derived from adipose tissue-derived stem cells (ADSCs). Method An experimental study was conducted with 18 hind legs of New Zealand rabbits, which were divided into three study groups according to the intervention to be performed. In group I, no surgical procedure was performed; in group II-SHAN, the experimental lesion was performed without any additional intervention protocol; in group III-Intervention, the addition of ADSCs was performed in the same topography of the experimental lesion. After the proposed period, 2 weeks, the material was collected and submitted to macroscopic and histological evaluation. Results The quantitative analysis showed that the addition of ADSCs is related to the reduction of inflammatory cells in the 2-week evaluation (164.2 cells in group II - SHAN to 89.62 cells in group III - ADSC). The qualitative analysis of the slides with Picrosirius red, noticed an increase in orange/yellow fibers in group III - ADSC, which evidences a final healing process. The macroscopic evaluation found no difference between the groups. Conclusion The use of ADSCs in the treatment of acute muscle injury presented histological advantages when compared to their non-use.

Keywords: mesenchymal stem cells; muscles; muscular diseases; regeneration; regenerative medicine.

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