Differential expression of circRNAs of testes with high and low sperm motility in Yili geese

Yingping Wu; Haiying Li; Xiaoyu Zhao; Gulnar Baki; Chen Ma; Yingying Yao; Jiahui Li; Yang Yao; Lin Wang

doi:10.3389/fgene.2022.970097

Differential expression of circRNAs of testes with high and low sperm motility in Yili geese

Front Genet. 2022 Sep 26:13:970097. doi: 10.3389/fgene.2022.970097. eCollection 2022.

Authors

Yingping Wu¹, Haiying Li¹, Xiaoyu Zhao¹, Gulnar Baki¹, Chen Ma¹, Yingying Yao¹, Jiahui Li¹, Yang Yao¹, Lin Wang¹

Affiliation

¹ College of Animal Science, Xinjiang Agricultural University, Urumqi, China.

Abstract

The aim of this study was to explore the potential biological function of circular RNAs (circRNAs) in the sperm motility traits of Xinjiang Yili geese, and to provide a reference for analyzing the mechanism of regulation of Yili geese sperm motility. The 10 selected Xinjiang Yili Geese with high or low sperm motility (five for each group) were 3 years old, in good health, and were kept in the same feeding conditions. Yili geese were slaughtered for the collection of testicular tissue and high-throughput sequencing technology was used to screen differentially expressed circRNAs for bioinformatics analysis. Combined with the previously screened miRNAs related to the sperm motility of Yili geese, the circRNAs miRNAs regulatory network was constructed. The results showed that a total of 26,311 circRNAs were obtained from testicular tissues with high and low sperm motility, and 173 DECs were screened between the two groups (p < 0.05, |log2Foldchange|>0), of which 82 were up-regulated and 91 were down-regulated. Functional analysis of the source genes of these DECs showed that the source genes were mainly involved in biological processes. KEGG enrichment analysis showed that the source genes of DECs were mainly enriched in autophagy-animal, ubiquinone and other terpenoid-quinone biosynthesis, progesterone-mediated oocyte maturation, regulation of the actin cytoskeleton and other pathways. Furthermore, the visual regulatory network of differential circRNA-miRNA-mRNA was constructed, including 20 circRNAs, 18 miRNAs and 177 mRNAs, and nine core regulatory circRNAs were screened, including novell_circ_0045314, novel_circ_0019994 and novel_circ_0020422, etc., targeting ppy-mir-16, hsa-mir-221-3p, gga-mir-499-5p, etc. The results suggest that circRNAs may interact with miRNAs to further regulate mRNA to regulate sperm motility in Yili geese, so as to provide a reference for analyzing the molecular mechanism of sperm motility regulation.

Keywords: circrnas; sperm motility; testis; transcriptomic; yili geese.