Comparative assessment of enamel remineralisation on the surface microhardness of demineralized enamel - an in vitro study

PeerJ. 2022 Oct 7:10:e14098. doi: 10.7717/peerj.14098. eCollection 2022.

Abstract

Objective: The main objective of the study was to compare two different remineralising materials containing casein phosphopeptide-amorphous calcium phosphate, bioactive glass on enamel surface microhardness.

Materials and methods: Thirty premolars were used for specimen preparation. Group 1 (the control group) consisted of intact enamel samples, group 2: CPP-ACPF (Tooth Mousse Plus), group 3: bioenamel remineralising gel (Prevest DenPro). All specimens were subjected to demineralisation except the control group, followed by which remineralising agents were applied. A universal hardness tester was used to assess the surface microhardness of all samples. Results were analysed using one-way ANOVA test and comparison was analysed using Scheffe's post hoc least significant difference (LSD) test.

Results: Both remineralising agents used in groups 2 and 3 have shown significant outcome in terms of improving the surface microhardness in comparison with the control group. Group 2 increased the enamel hardness by 8.34 where P = 0.023 whereas group 3 increased the hardness by 5.87, where P = 0.01.

Conclusion: Group 2 has a superior hardness value than group 3; however, no statistically significant results were obtained between both the groups.

Keywords: Bioglass; Calcium phosphate; Carious lesions; Demineralisation; Fluoride; Hydroxyapatite; Microhardness; Remineralisation.

MeSH terms

  • Control Groups
  • Dental Enamel
  • Hardness
  • Research Design*
  • Tooth Remineralization* / methods

Grants and funding

The authors received no funding for this work.