Single-Pair and Small-Group Crosses of Aedes aegypti

Margot P Wohl; Conor J McMeniman

doi:10.1101/pdb.prot108018

Single-Pair and Small-Group Crosses of Aedes aegypti

Cold Spring Harb Protoc. 2023 Mar 1;2023(3):108018-pdb.prot. doi: 10.1101/pdb.prot108018.

Authors

Margot P Wohl¹, Conor J McMeniman^{2

3}

Affiliations

¹ W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA.
² W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA cmcmeni1@jhu.edu.
³ The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

Abstract

Aedes aegypti is an emerging model insect species used in genetics studies because of its ease of laboratory rearing, desiccation-resistant eggs, expanding genetic toolkit, and high-quality reference genome. Here, we describe procedures to isolate and sex virgin female and male mosquitoes and establish successful mating crosses. We also detail how to blood feed mosquitoes from these crosses, isolate individual or small groups of females for egg laying, condition these eggs for storage and hatching, and verify female mating status.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Aedes*
Animals
Female
Laboratories
Male

Abstract

Publication types

MeSH terms

Grants and funding