Batch Rearing Aedes aegypti

Margot P Wohl; Conor J McMeniman

doi:10.1101/pdb.prot108017

Batch Rearing Aedes aegypti

Cold Spring Harb Protoc. 2023 Mar 1;2023(3):108017-pdb.prot. doi: 10.1101/pdb.prot108017.

Authors

Margot P Wohl¹, Conor J McMeniman^{2

3}

Affiliations

¹ W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA.
² W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA cmcmeni1@jhu.edu.
³ The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

Abstract

Standardized rearing methods for the yellow fever mosquito Aedes aegypti are critical to facilitate controlled laboratory studies. This protocol describes a batch rearing protocol for Aedes aegypti stocks that yields healthy eggs, larvae, pupae, and adults in the laboratory for long-term colony maintenance and experimental manipulation. Foundational principles for the rearing and containment of these life cycle stages, as well as steps for mating and blood feeding Aedes aegypti to yield viable eggs for continuous culture or storage, are detailed.

Batch Rearing Aedes aegypti

Authors

Affiliations

Abstract

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Grants and funding