Simultaneous production of cellobiose and xylobiose from alkali-treated bagasse using cellulase secreted by Fe-ion-irradiated Trichoderma reesei mutant

Abstract

Cellobiose and xylobiose are disaccharides composed of two glucose or xylose units with β-1,4 linkages. This study aimed to isolate a Trichoderma reesei mutant that lacks β-glucosidase and β-xylosidase activities for the simultaneous production of these disaccharides. Mutagenesis using Fe-ion beam resulted in a mutant strain, T. reesei T1640; the cellulase production in this strain was as high as that in the parent strain. Genomic analysis revealed that T1640 lost both the β-glucosidase and β-xylosidase activities owing to the translocation of the responsible genes. Hydrolysis of alkali-treated bagasse using the enzymes from T1640 leads to high yields (365 mg/g-biomass) and ratios (72.7% of the total sugars) of cellobiose and xylobiose.

Keywords: Cellobiose; Cellulase; Ion-beam irradiation; Trichoderma reesei; Xylobiose.