Effect of Fe0 particle size on buffering characteristics and biohydrogen production in high-load photo fermentation system of corn stover

Shengnan Zhu; Yang Zhang; Zhiping Zhang; Yameng Li; Fuke Ai; Quanguo Zhang

doi:10.1016/j.biortech.2022.128086

Effect of Fe⁰ particle size on buffering characteristics and biohydrogen production in high-load photo fermentation system of corn stover

Bioresour Technol. 2022 Nov:364:128086. doi: 10.1016/j.biortech.2022.128086. Epub 2022 Oct 8.

Authors

Shengnan Zhu¹, Yang Zhang¹, Zhiping Zhang¹, Yameng Li¹, Fuke Ai¹, Quanguo Zhang²

Affiliations

¹ Key Laboratory of New Materials and Facilities for Rural Renewable Energy (MOA of China), Henan Agricultural University, Zhengzhou 450002, China.
² Key Laboratory of New Materials and Facilities for Rural Renewable Energy (MOA of China), Henan Agricultural University, Zhengzhou 450002, China. Electronic address: zquanguo@163.com.

PMID: 36216289
DOI: 10.1016/j.biortech.2022.128086

Abstract

The aim of this work was to study the effects of Fe⁰ particle sizes (700 nm, 100 nm and 50 nm) addition on biohydrogen production, liquid culture characteristics and photosynthetic bacterial respond in the high-load photo fermentation system of corn stover within the concentration range of 200-1500 mg/L. Results showed that Fe⁰ with particle size of 700 nm had a better promotion effect on hydrogen production than 100 nm and 50 nm. The highest hydrogen yield of 74.32 ± 3.48 mL/g TS and hydrogen production rate of 3.31 ± 0.11 mL/g·h TS corn stover were obtained at 1000 and 1500 mg/L Fe⁰-700 nm, which were significantly increased by 92.88 % and 133.88 % compared with the control group. Further analysis indicated that Fe⁰ addition effectively alleviated pH drop, enhanced nitrogenase activity, promoted cell growth, and accelerated the consumption of acetic acid and butyric acid.

Keywords: Biohydrogen; Corn stover; Fe(0) particle size; High-load photo fermentation.