Screening of microalgae species and evaluation of algal-lipid stimulation strategies for biodiesel production

Yanan Yang; Shuhan Ge; Yitong Pan; Weiyi Qian; Shengnan Wang; Jian Zhang; Lin-Lan Zhuang

doi:10.1016/j.scitotenv.2022.159281

Screening of microalgae species and evaluation of algal-lipid stimulation strategies for biodiesel production

Sci Total Environ. 2023 Jan 20;857(Pt 1):159281. doi: 10.1016/j.scitotenv.2022.159281. Epub 2022 Oct 7.

Authors

Yanan Yang¹, Shuhan Ge¹, Yitong Pan¹, Weiyi Qian¹, Shengnan Wang¹, Jian Zhang², Lin-Lan Zhuang³

Affiliations

¹ Shandong Key Laboratory of Water Pollution Control and Resource Reuse and Shandong Key Laboratory of Environmental Processes and Health, School of Environmental Science & Engineering, Shandong University, Qingdao 266237, China.
² College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266590, China; Shandong Key Laboratory of Water Pollution Control and Resource Reuse and Shandong Key Laboratory of Environmental Processes and Health, School of Environmental Science & Engineering, Shandong University, Qingdao 266237, China.
³ Shandong Key Laboratory of Water Pollution Control and Resource Reuse and Shandong Key Laboratory of Environmental Processes and Health, School of Environmental Science & Engineering, Shandong University, Qingdao 266237, China. Electronic address: zhuanglinlan@sdu.edu.cn.

PMID: 36216060
DOI: 10.1016/j.scitotenv.2022.159281

Abstract

Microalgae is considered an alternative source for biodiesel production producing renewable, sustainable and carbon-neutral energy. Microalgae property changes among species, which determines the efficiency of biodiesel production. Besides the lipid content evaluation, multi-principles (including high lipid productivity, high biomass yield, pollution resistance and desired fatty acid, etc.) for superior oil-producing species screening was proposed in this review and three microalgae species (Chlorella vulgaris, Scenedesmus obliquus and Mychonastes afer) with high bio-lipid producing prospect were screened out based on big data digging and analysis. The multilateral strategies for algal-lipid stimulating were also compared, among which, nutrient restriction, temperature control, heterotrophy and chemicals addition showed high potential in enhancing lipid accumulation; while electromagnetic field showed little effect. Interestingly, it was found that the lipid accumulation was more sensitive to nitrogen (N)-limitation other than phosphorus (P). Nutrient restriction, salinity stress etc. enhanced lipid accumulation by creating a stressed environment. Hence, optimum conditions (e.g. N:15-35 mg/L and P:4-16 mg/L) should be set to balance the lipid accumulation and biomass growth, and further guarantee the algal-lipid productivity. Otherwise, two-step cultivation could be applied during all the stressed stimulation. Different from lab study, effectiveness, operability and economy should be all considered for stimulation strategy selection. Nutrient restriction, temperature control and heterotrophy were highly feasible after the multidimensional evaluation.

Keywords: Biodiesel production; Environmental stimulation; Lipid accumulation; Microalgae species; Nutrient restriction.

Publication types

Review

MeSH terms

Biofuels
Biomass
Chlorella vulgaris*
Fatty Acids
Microalgae*

Substances

Biofuels
Fatty Acids