In order to study the influence and mechanisms of color differences using 3D-shaped objects, 440 pairs of 3D samples surrounding five CIE color centers (gray, red, yellow, green, and blue) with the variations of gloss, size, and shape were prepared by a Sailner 3D color printer, and their color differences were assessed by 26∼45 observers using the gray-scale method. The new color difference data were used to investigate the parametric effects (gloss, 3D shape, and size) on the perceived color difference. Results indicate that, for 3D objects, high gloss and small size objects (2 cm) raise smaller visual color differences than matte and large size objects (4 cm), and the visual color difference of spheres is larger than that of the cone and cylinder sample pairs. The chromaticity ellipses indicated that the glossy samples with different shapes will arouse fairly different visual perceptions, especially for sphere and cylinder samples.