Anti-inflammatory compounds from the mangrove endophytic fungus Amorosia sp. SCSIO 41026

Xue Ren; Chunmei Chen; Yuxiu Ye; Ziying Xu; Qingliang Zhao; Xiaowei Luo; Yonghong Liu; Peng Guo

doi:10.3389/fmicb.2022.976399

Anti-inflammatory compounds from the mangrove endophytic fungus Amorosia sp. SCSIO 41026

Front Microbiol. 2022 Aug 29:13:976399. doi: 10.3389/fmicb.2022.976399. eCollection 2022.

Authors

Xue Ren¹, Chunmei Chen^{2

3}, Yuxiu Ye⁴, Ziying Xu¹, Qingliang Zhao⁵, Xiaowei Luo⁴, Yonghong Liu^{2

3

4

6}, Peng Guo¹

Affiliations

¹ Capital Institute of Pediatrics, Beijing, China.
² CAS Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.
³ University of Chinese Academy of Sciences, Beijing, China.
⁴ Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning, China.
⁵ State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Center for Molecular Imaging and Translational Medicine, Department of Laboratory Medicine, School of Public Health, Xiamen University, Xiamen, China.
⁶ Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, China.

Abstract

Three new chlorinated compounds, including two propenylphenol derivatives, chlorophenol A and B (1 and 2), and one benzofuran derivative, chlorophenol C (3), together with 16 known compounds, were isolated from the mangrove endophytic fungus Amorosia sp. SCSIO 41026. 7-Chloro-3,4-dihydro-6,8-dihydroxy-3-methylisocoumarine (4) and 2,4-dichloro-3-hydroxy-5-methoxy-toluene (5) were obtained as new natural products. Their structures were elucidated by physicochemical properties and extensive spectroscopic analysis. Compounds 1, 4, 7, 9, 13, 15, 16, and 19 possessed inhibitory effects against the excessive production of nitric oxide (NO) and pro-inflammatory cytokines in lipopolysaccharide (LPS)-challenged RAW264.7 macrophages without obvious cytotoxicity. Moreover, 5-chloro-6-hydroxymellein (13) further alleviated the pathological lung injury of LPS-administrated mice and protected RAW264.7 macrophages against LPS-induced inflammation through PI3K/AKT pathway in vivo. Our research laid the foundation for the application of compound 13 as a potential anti-inflammatory candidate.

Keywords: Amorosia sp.; acute lung injury mice; anti-inflammatory activity; chlorinated compounds; marine fungi.