Multifaceted analyses reveal carbohydrate metabolism mainly affecting the quality of postharvest bamboo shoots

Zhen Li; Xiurong Xu; Kebin Yang; Chenglei Zhu; Yan Liu; Zhimin Gao

doi:10.3389/fpls.2022.1021161

Multifaceted analyses reveal carbohydrate metabolism mainly affecting the quality of postharvest bamboo shoots

Front Plant Sci. 2022 Sep 21:13:1021161. doi: 10.3389/fpls.2022.1021161. eCollection 2022.

Authors

Zhen Li^{1

2}, Xiurong Xu^{1

2

3}, Kebin Yang^{1

2}, Chenglei Zhu^{1

2}, Yan Liu^{1

2}, Zhimin Gao^{1

2}

Affiliations

¹ International Centre for Bamboo and Rattan, Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, Beijing, China.
² Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, Beijing, China.
³ Zhejiang Academy of Forestry, Hangzhou, China.

Abstract

Bamboo shoot is one of nutritious vegetables in China. However, the edible quality of fresh bamboo shoots deteriorates easily after harvest. Here, morphological, physiological, transcriptomic and microRNA sequencing analyses were conducted to investigate the postharvest characteristics of moso bamboo (Phyllostachys edulis) shoots. Rapid decreases of soluble sugars, structural polysaccharides and hydrolyzed tannins, and increases of lignin and condensed tannins were observed in the postharvest bamboo shoots. Differentially expressed genes (DEGs) and miRNAs with opposite trends were mainly enriched in structural polysaccharide metabolism, starch and sucrose metabolism and glycolysis pathways, which were consistent with the changes of carbohydrates. A co-expression network of carbohydrate metabolism was constructed, which was verified by qPCR and yeast one-hybrid (Y1H) assay. Furthermore, the function of one hub glycosyltransferase gene was validated in Arabidopsis, which confirmed that it was involved in xylan biosynthesis. These results are of great significance for revealing the carbohydrate metabolism mechanisms of postharvest bamboo shoots and provide a potential candidate gene for molecular breeding related to xylan in the future.

Keywords: Phyllostachys edulis shoot; carbohydrate metabolism; co-expression network; postharvest physiology; xylan biosynthesis.