Quantitative genetic analysis reveals potential to breed for improved white clover growth in symbiosis with nitrogen-fixing Rhizobium bacteria

Sean K Weith; M Z Zulfi Jahufer; Rainer W Hofmann; Craig B Anderson; Dongwen Luo; O Grace Ehoche; Greig Cousins; E Eirian Jones; Ross A Ballard; Andrew G Griffiths

doi:10.3389/fpls.2022.953400

Quantitative genetic analysis reveals potential to breed for improved white clover growth in symbiosis with nitrogen-fixing Rhizobium bacteria

Front Plant Sci. 2022 Sep 20:13:953400. doi: 10.3389/fpls.2022.953400. eCollection 2022.

Authors

Affiliations

¹ AgResearch, Grasslands Research Centre, Palmerston North, New Zealand.
² Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, New Zealand.
³ PGG Wrightson Seeds Ltd., Grasslands Research Centre, Palmerston North, New Zealand.
⁴ South Australian Research and Development Institute (SARDI), Adelaide, SA, Australia.

Abstract

White clover (Trifolium repens) is integral to mixed pastures in New Zealand and temperate agriculture globally. It provides quality feed and a sustainable source of plant-available nitrogen (N) via N-fixation through symbiosis with soil-dwelling Rhizobium bacteria. Improvement of N-fixation in white clover is a route to enhancing sustainability of temperate pasture production. Focussing on seedling growth critical for crop establishment and performance, a population of 120 half-sibling white clover families was assessed with either N-supplementation or N-fixation via inoculation with a commercial Rhizobium strain (TA1). Quantitative genetic analysis identified significant (p < 0.05) family additive genetic variance for Shoot and Root Dry Matter (DM) and Symbiotic Potential (SP), and Root to Shoot ratio. Estimated narrow-sense heritabilities for above-ground symbiotic traits were moderate (0.24-0.33), and the strong (r ≥ 0.97) genetic correlation between Shoot and Root DM indicated strong pleiotropy or close linkage. The moderate (r = 0.47) phenotypic correlation between Shoot DM under symbiosis vs. under N-supplementation suggested plant growth with mineral-N was not a strong predictor of symbiotic performance. At 5% among-family selection pressure, predicted genetic gains per selection cycle of 19 and 17% for symbiotic traits Shoot DM and Shoot SP, respectively, highlighted opportunities for improved early seedling establishment and growth under symbiosis. Single and multi-trait selection methods, including a Smith-Hazel index focussing on an ideotype of high Shoot DM and Shoot SP, showed commonality of top-ranked families among traits. This study provides a platform for proof-of-concept crosses to breed for enhanced seedling growth under Rhizobium symbiosis and is informative for other legume crops.

Keywords: Rhizobium leguminosarum bv. trifolii TA1; Trifolium repens (white clover); genetic gain; nitrogen fixation; plant-microbe interactions; quantitative genetics and breeding; symbiosis.